信號素4D(SEMA4D)重組蛋白
Recombinant Semaphorin 4D (SEMA4D)
CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D
- 編號RPB430Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞膜
- 預測分子量16.2kDa
- 實際分子量18kDa(差異分析請參閱說明書)
- 片段與標簽Phe502~Phe636 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點8.8
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 984 ¥ 2460 ¥ 4920 ¥ 14760 ¥ 36900
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產品包裝(模擬)
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產品包裝(模擬)
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Gene sequencing
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB430Hu01 | 信號素4D(SEMA4D)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB430Hu01 | 信號素4D(SEMA4D)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB430Hu22 | 信號素4D(SEMA4D)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB430Hu | 信號素4D(SEMA4D)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB430Hu | 信號素4D(SEMA4D)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Expert Opin Ther Targets. | Circulating semaphorin-4D and plexin-B1 levels in postmenopausal women with low bone mass: the 3-month effect of zoledronic acid, denosumab or teriparatide treatment [Pubmed:25395071] |
| Obesity (Silver Spring). | Genome-wide analysis identifies an african-specific variant in SEMA4D associated with body mass index. [pubmed:28296344] |
| Clinical Lymphoma, Myeloma & Leukemia | A Revised Staging System for Waldenstr?m's Macroglobulinemia [10.1016/j.clml.2017.07.146] |
| Clinical Lymphoma, Myeloma & Leukemia | Extramedullary Multiple Myeloma: Clinical Features and Prognosis in Argentina [10.1016/j.clml.2017.07.144] |
| Blood?Cancer?Journal | Semaphorin 4D correlates with increased bone resorption, hypercalcemia, and disease stage in newly diagnosed patients with multiple myeloma [Pubmed:29748532] |
| Analytical Biochemistry | A high-sensitivity enzyme immunoassay for the quantification of soluble human semaphorin 4D in plasma [Pubmed: 30879960] |
| Journal of Allergy and Clinical Immunology-In Practice | Pathological and therapeutic implications of eosinophil-derived semaphorin 4D in eosinophilic chronic rhinosinusitis [Pubmed: 32035658] |
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