表面活性物質關聯蛋白B(SP-B)重組蛋白
Recombinant Surfactant Protein B (SP-B)
SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein
- 編號RPB622Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌, 細胞外基質
- 預測分子量20.4kDa
- 實際分子量20kDa(差異分析請參閱說明書)
- 片段與標簽Gly20~Pro193 with N-terminal His Tag
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 95%
- 等電點5.8
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 960 ¥ 2400 ¥ 4800 ¥ 14400 ¥ 36000
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產品包裝(模擬)
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產品包裝(模擬)
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB622Mu01 | 表面活性物質關聯蛋白B(SP-B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB622Mu01 | 表面活性物質關聯蛋白B(SP-B)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB622Mu71 | 表面活性物質關聯蛋白B(SP-B)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEB622Mu | 表面活性物質關聯蛋白B(SP-B)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB622Mu | 表面活性物質關聯蛋白B(SP-B)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Respiratory Physiology & Neurobiology | Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery [PubMed: 21736957] |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps [PubMed: 23406594] |
| 19 | Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study [PubMed: 20650982] |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins [PubMed: PMC3551896] |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS [PubMed: PMC3787032] |
| Cancer Cell. | Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models [PubMed: PMC3406925] |
| Respir Physiol Neurobiol | Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project [Pubmed: 23619193] |
| PLOS ONE | Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure [Pubmed:25514679] |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge [Opus4:Source] | |
| Int J Cardiol | Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study [PubMed: 26310985] |
| Plos One | Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) [Pubmed:27337142] |
| PLoS One | The?Cerebral?Surfactant?System?and Its?Alteration?in?HydrocephalicConditions. [pubmed:27656877] |
| Frontiers in Aging Neuroscience | Correlations?of?Ventricular?Enlargement?with?Rheologically?Active?SurfactantProteins?in?Cerebrospinal?Fluid. [pubmed:28101052] |
| Frontiers in?Aging?Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid [PMC5209370] |
| Respiratory Physiology & Neurobiology | Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers [pubmed:28467885] |
| Cellular Physiology and Biochemistry | Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production [pubmed:28743125] |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. [pubmed:29167126] |
| Respiratory research | Effects and molecular mechanisms of intrauterine infection/inflammation on lung development [Pubmed:29747649] |
| Respiratory Physiology & Neurobiology? | Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan [Pubmed:29548887] |
| Lung? | Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation [Pubmed:29445934] |
| American?Journal?of?Physiology?Lung?Cellular?andMolecular?Physiology | Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells [Pubmed: 30211653] |
| Respiratory?Research | Culture of human alveolar epithelial type II cells by sprouting [Pubmed: 30340591] |
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