親環素A(CYPA)多克隆抗體
Polyclonal Antibody to Cyclophilin A (CYPA)
PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein
- 編號PAA979Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA979Hu01-親環素A(CYPA)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CYPA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human CYPA Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human CYPA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Testis Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human CYPA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human CYPA Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant CYPA, Human.
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Western Blot; Sample: Human Liver lysate;
Primary Ab: 4μg/ml Rabbit Anti-Human CYPA Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Serum;
Primary Ab: 4μg/ml Rabbit Anti-Human CYPA Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Serum;
Primary Ab: 4μg/ml Rabbit Anti-Human CYPA Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Cerebrum lysate; Lane3: HepG2 cell lysate; Lane4: Jurkat cell lysate
Primary Ab: 0.05μg/ml Rabbit Anti-Human CYPA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Mouse Testis lysate; Lane2: Mouse Cerebrum lysate; Lane3: Rat Lung lysate; Lane4: Hela cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human CYPA Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human CYPA Polyclonal Antibody (Catalog PAA979Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對CYPA的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別CYPA。
用法
Western blotting: 0.5-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA979Hu01 | 親環素A(CYPA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA979Hu21 | 親環素A(CYPA)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAA979Hu08 | 親環素A(CYPA)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA979Hu01 | 親環素A(CYPA)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| LAA979Hu81 | 親環素A(CYPA)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA979Hu71 | 親環素A(CYPA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA979Hu22 | 親環素A(CYPA)單克隆抗體 | WB,IHC,ICC/IF |
| MAA979Hu23 | 親環素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Hu24 | 親環素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Hu25 | 親環素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Hu21 | 親環素A(CYPA)單克隆抗體 | WB; IHC |
| FAA979Hu02 | 抗親環素A(CYPA)單克隆抗體 | Flow cytometry. |
| FAA979Hu06 | 抗親環素A(CYPA)單克隆抗體 | Flow cytometry. |
| FAA979Hu08 | 抗親環素A(CYPA)單克隆抗體 | Flow cytometry. |
| LAA979Hu72 | 親環素A(CYPA)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA979Hu | 親環素A(CYPA)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA979Hu | 親環素A(CYPA)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| PSA979Hu01 | 親環素A(CYPA)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA979Hu01 | 親環素A(CYPA)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Atherosclerosis | EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation [PubMed: 19758589] |
| Biomarkers | Cyclophilin-A: a novel biomarker for untreated male essential hypertension [Pubmed: 24164594] |
| Cardiovascular Diabetology | Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease [Pubmed:24502618] |
| International Journal of Biological Sciences | Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction [Pubmed:25552928] |
| Medicine | Cyclophilin A in Ruptured Intracranial Aneurysm [PubMed: 26426668] |
| Mol Cell Biochem | PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease [PubMed: 26702934] |
| Medicine (Baltimore) | Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus [PubMed: 26496315] |
| International Journal of Cardiology | Cyclophilin?A?modulates?bone?marrow-derived?CD117(+)?cells?and?enhances?ischemia-induced?angiogenesisvia?the?SDF-1/CXCR4?axis. [Pubmed:27057951] |
| Clinica?Chimica?Acta | Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor [pubmed:27823952] |
| Cardiovascular?Diabetology | Cyclophilin?A?enhances?macrophage?differentiation?and?lipid?uptake?in?high?glucose?conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. [pubmed:27809851] |
| Clinical?Science | Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity [Pubmed:29382697] |
| Journal of Molecular Histology | Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitis [Pubmed:29318411] |
| Free?Radical?Research | Cyclophilin A and CD147 associate with progression of diabetic nephropathy [Pubmed: 30572748] |
| The Kaohsiung Journal of Medical Sciences | Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarction [Pubmed: 31670477] |
| Cells. | Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients [Pubmed: 31936351] |
| JAMA Neurology | APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers?… [Pubmed: 24030206] |
| Menoufia Medical Journal | Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy [] |
| Toxins | Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy [34437430] |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death [Pubmed:35732625] |
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