干擾素調節因子3(IRF3)多克隆抗體
Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)
- 編號PAB589Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPB589Hu01-干擾素調節因子3(IRF3)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 598 ¥ 1394 ¥ 1992 ¥ 4980 ¥ 19920
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產品包裝(模擬)
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產品包裝(模擬)
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Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
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Figure: FITC staining on IHC-P;
Sample: Hela cells. -
AF488 staining on IF;
Sample: Hepa1-6 cell
Primary Ab: 20μg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11) -
AF488 staining on IF;
Sample: NIH/3T3 cell
Primary Ab: 20μg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Western Blot; Sample: Lane1: 3T3-L1 cell lysate; Lane2: Rat Kidney lysate Primary Ab: 0.6μg/ml Rabbit Anti-Human IRF3 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 3μg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Hela cell lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human IRF3 Polyclonal Antibody (Catalog PAB589Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對IRF3的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別IRF3。
用法
Western blotting: 0.01-3μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-30μg/mL
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB589Hu01 | 干擾素調節因子3(IRF3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB589Hu01 | 干擾素調節因子3(IRF3)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| MAB589Hu22 | 干擾素調節因子3(IRF3)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB589Hu | 干擾素調節因子3(IRF3)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB589Hu | 干擾素調節因子3(IRF3)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PLOS?Pathogens | RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production [pubmed:29186193] |
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