M2-型丙酮酸激酶(PKM2)多克隆抗體
Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle
- 編號PAA588Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA588Ra01-M2-型丙酮酸激酶(PKM2)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 616 ¥ 1436 ¥ 2052 ¥ 5130 ¥ 20520
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Samples: Rat Testis Tissue; Primary Ab: 10μg/ml Rabbit Anti-Rat PKM2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Intestine Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Stomach Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Spleen Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat PKM2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat PKM2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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WB圖
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Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-rat PKM2 Polyclonal Antibody (Catalog # PAA588Ra01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對PKM2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別PKM2。
用法
免疫印跡：0.2-2μg/mL;1:250-2500
免疫組織化學：5-20μg/mL;1:25-100
免疫細胞化學：5-20μg/mL;1:25-100
最佳稀釋倍數最終由用戶決定。
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA588Ra01 | M2-型丙酮酸激酶(PKM2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA588Ra01 | M2-型丙酮酸激酶(PKM2)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA588Ra01 | 抗M2-型丙酮酸激酶(PKM2)多克隆抗體 | Flow cytometry. |
| MAA588Ra21 | M2-型丙酮酸激酶(PKM2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA588Ra | M2-型丙酮酸激酶(PKM2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA588Ra | M2-型丙酮酸激酶(PKM2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Cancer Research | Antigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3 [AACR: 70189] |
| Molecular BioSystems | Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer [Pubmed:25483567] |
| Tumour Biol | M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells [PubMed: 26404132] |
| Oncotarget | Identification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitis [Pubmed:29707118] |
| Journal of?Neurotrauma | Acteoside improves muscle atrophy and motor function by inducing new myokine secretion in chronic spinal cord injury [Pubmed: 30318996] |
| Cell Reports | Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway [Pubmed: 32049010] |
| Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) [] | |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins [] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212] |
| Life (Basel) | Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib [34947916] |
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