谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體
Polyclonal Antibody to Transglutaminase 2 (TGM2)
tTG; TG2; C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase; Transglutaminase 2, Tissue; Transglutaminase C; Transglutaminase H
- 編號PAB830Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB830Hu01-谷氨酰胺轉(zhuǎn)胺酶2(TGM2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 598 ¥ 1394 ¥ 1992 ¥ 4980 ¥ 19920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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DAB staining on IHC-P;<br/>Sample: Human Uterus Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Human Placenta Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Human Lymph node Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Human Spleen Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Human Uterus Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant TGM2, Human.
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Western Blot; Sample: Mouse Placenta lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: TGM2 knockout Hela cell lysate;
Predicted MW: 77kd
Observed MW: 77kd
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Placenta lysate; Lane3: HepG2 cell lysate; Lane4: K562 cell lysate; Lane5: Hela cell lysate
Primary Ab: 0.1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對TGM2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別TGM2。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體 | WB,IHC,ICC/IF |
| LAB830Hu71 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB830Hu22 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)單克隆抗體 | WB; IHC; ICC; IP. |
| MEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
| CGB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)cDNA | PCR、蛋白表達(dá)等 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Connective Tissue Research | Tissue transglutaminase is involved in mechanical load–induced osteogenic differentiation of human ligamentum flavum cells [Pubmed:27115725] |
| American Journal of Respiratory Cell and Molecular Biology | ECM Crosslinking Enhances Fibroblast Growth and Protects Against Matrix Proteolysis in Lung Fibrosis. [pubmed:29053339] |
| 3 Biotech | Metformin attenuates TGF?β1?induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF?β pathways [Pubmed: 32550106] |
