晚期糖基化終末產物(AGE)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Advanced Glycation End Product (AGE)
AGEs; Advanced Glycation End Products
- 編號CEB353Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍98.8-8,000ng/mL
- 靈敏度最小可檢測劑量小于等于38.2ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2985 ¥ 4264 ¥ 19186 ¥ 36241 ¥ 298452
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測晚期糖基化終末產物(AGE)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的晚期糖基化終末產物(AGE)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-93 | 89 |
| EDTA plasma(n=5) | 85-95 | 91 |
| heparin plasma(n=5) | 87-103 | 98 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的晚期糖基化終末產物(AGE)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中晚期糖基化終末產物(AGE)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-101% | 79-98% | 98-105% | 90-102% |
| EDTA plasma(n=5) | 99-105% | 80-101% | 81-90% | 95-104% |
| heparin plasma(n=5) | 82-98% | 89-101% | 87-103% | 80-95% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數。
實驗原理
本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將晚期糖基化終末產物(AGE)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物，然后加入HRP標記的親和素，經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關產品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| NPB353Ge01 | 晚期糖基化終末產物(AGE)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB353Ge01 | 晚期糖基化終末產物(AGE)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB353Ge01 | 晚期糖基化終末產物(AGE)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB353Ge71 | 晚期糖基化終末產物(AGE)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAB353Ge81 | 晚期糖基化終末產物(AGE)多克隆抗體(異硫氰酸熒光素標記) | IHC; ICC;ELISA |
| MAB353Ge21 | 晚期糖基化終末產物(AGE)單克隆抗體 | WB; IHC; ICC; IP. |
| HEB353Ge | 晚期糖基化終末產物(AGE)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB353Ge | 晚期糖基化終末產物(AGE)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB353Ge | 晚期糖基化終末產物(AGE)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSB353Ge11 | 晚期糖基化終末產物(AGE)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
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