異檸檬酸脫氫酶1(IDH1)單克隆抗體
Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)
PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase
- 編號MAH839Hu23
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C13
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPH839Hu01-異檸檬酸脫氫酶1(IDH1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 688 ¥ 1604 ¥ 2292 ¥ 5730 ¥ 22920
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 30μg/ml Mouse Anti-Human IDH1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Prostate Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human IDH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human IDH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Glioma Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human IDH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human IDH1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Prostate Tissue
Primary Ab: 30μg/ml Mouse Anti-Human IDH1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 30μg/ml Mouse Anti-Human IDH1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Human Liver lysate; Lane2: Porcine Liver lysate Primary Ab: 5μg/ml Mouse Anti-Human IDH1 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對IDH1的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別IDH1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPH839Hu01 | 異檸檬酸脫氫酶1(IDH1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH839Hu01 | 異檸檬酸脫氫酶1(IDH1)多克隆抗體 | WB,IHC |
| FAH839Hu01 | 抗異檸檬酸脫氫酶1(IDH1)多克隆抗體 | Flow cytometry. |
| MAH839Hu22 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC |
| MAH839Hu23 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAH839Hu25 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAH839Hu27 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAH839Hu29 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAH839Hu24 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC |
| MAH839Hu26 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC |
| MAH839Hu21 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
| RAH839Hu21 | 異檸檬酸脫氫酶1(IDH1)重組抗體 | WB; IFC; IHC; IP. |
| FAH839Hu02 | 抗異檸檬酸脫氫酶1(IDH1)單克隆抗體 | Flow cytometry. |
| FAH839Hu04 | 抗異檸檬酸脫氫酶1(IDH1)單克隆抗體 | Flow cytometry. |
| FAH839Hu06 | 抗異檸檬酸脫氫酶1(IDH1)單克隆抗體 | Flow cytometry. |
| FAH839Hu08 | 抗異檸檬酸脫氫酶1(IDH1)單克隆抗體 | Flow cytometry. |
| LAH839Hu76 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| IEH839Hu | 異檸檬酸脫氫酶1(IDH1)檢測試劑盒(酶聯免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEH839Hu | 異檸檬酸脫氫酶1(IDH1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCH839Hu | 異檸檬酸脫氫酶1(IDH1)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMH839Hu | 異檸檬酸脫氫酶1(IDH1)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| PSH839Hu01 | 異檸檬酸脫氫酶1(IDH1)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSH839Hu01 | 異檸檬酸脫氫酶1(IDH1)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Molecular & Cellular Proteomics | Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic Analysis [Mcponline: 008821] |
| Molecular & Cellular Proteomics | Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic Analysis [Pubmed: 22064513] |
| Clinical Cancer Research | Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancer [Pubmed: 24046070] |
| Onco Targets Ther | The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells [PubMed: 25914546] |
| Oncogene | Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanism [Pubmed:29921847] |
| Molecular Oncology | IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancer [Pubmed:29465809] |
| Cancers | Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver … [Pubmed: 32443439] |
| International Journal of Cancer | Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer [Pubmed:35689436] |
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