CD73分子(CD73)多克隆抗體
Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)
NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto
- 編號PAB250Bo01
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPB250Bo01-CD73分子(CD73)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 716 ¥ 1672 ¥ 2388 ¥ 5970 ¥ 23880
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產品包裝(模擬)
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產品包裝(模擬)
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Figure. Western Blot; Sample: Recombinant NT5E, Bovine.
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Western Blot; Sample: Bovine Cerebrum lysate;
Primary Ab: 3μg/ml Rabbit Anti-Bovine NT5E Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type K562 cell lysate;
Lane 2: NT5E knockout K562 cell lysate;
Predicted MW: 63kd
Observed MW: 70kd
Primary Ab: 3μg/ml Rabbit Anti-Bovine NT5E Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Bovine Liver lysate;
Primary Ab: 3μg/ml Rabbit Anti-Bovine NT5E Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human K562 cell lysate;
Primary Ab: 3μg/ml Rabbit Anti-Bovine NT5E Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對CD73的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別CD73。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB250Bo01 | CD73分子(CD73)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB250Bo01 | CD73分子(CD73)多克隆抗體 | WB; IHC; ICC; IP. |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Autoimmunity | The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation [Pubmed:25640206] |
| Talanta | Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment?… [10.1016:j.talanta.2018.01.044] |
| Cancer?Biomarkers | Expression and clinical significance of serum NT5E protein in patients with colorectal cancer [Pubmed: 30932882] |
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