細胞外信號調(diào)節(jié)激酶2(ERK2)多克隆抗體
Polyclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2)
MAPK1; P38; P40; ERK2; ERT1; MAPK2; P42MAPK; P42-MAPK; PRKM1; PRKM2; P41; P41mapk; P41-mapk; Mitogen-Activated Protein Kinase 1; Mitogen-activated protein kinase 2
- 編號PAA930Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA930Hu02-細胞外信號調(diào)節(jié)激酶2(ERK2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
- 應用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 662 ¥ 1546 ¥ 2208 ¥ 5520 ¥ 22080
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Porcine Small intestine Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Samples: Porcine Liver Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Skeletal muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20μg/ml Rabbit Anti-Human IGF1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant ERK2, Human.
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Western Blot Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Canine Cerebrum lysate; Lane2: Gallus Cerebrum lysate; Lane3: Bovine Cerebrum lysate; Lane4: Cavia Cerebrum lysate; Lane5: Caprine Cerebrum lysate
Primary Ab: 0.05μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human ERK2 Polyclonal Antibody (Catalog PAA930Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對ERK2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別ERK2。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA930Hu01 | 細胞外信號調(diào)節(jié)激酶2(ERK2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA930Hu01 | 細胞外信號調(diào)節(jié)激酶2(ERK2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA930Hu02 | 細胞外信號調(diào)節(jié)激酶2(ERK2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA930Hu01 | 細胞外信號調(diào)節(jié)激酶2(ERK2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA930Hu02 | 細胞外信號調(diào)節(jié)激酶2(ERK2)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| CAA930Hu01 | 抗細胞外信號調(diào)節(jié)激酶2(ERK2)多克隆抗體 | Loading Control of WB |
| MAA930Hu23 | 細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA930Hu22 | 細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | WB; IHC |
| MAA930Hu27 | 細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA930Hu26 | 細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA930Hu28 | 細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA930Hu24 | 細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | WB; IHC; ICC; IP. |
| CAA930Hu22 | 抗細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | Loading Control of WB |
| MAA930Hu25 | 細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA930Hu21 | 細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA930Hu04 | 抗細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | Flow cytometry. |
| FAA930Hu06 | 抗細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | Flow cytometry. |
| FAA930Hu02 | 抗細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | Flow cytometry. |
| FAA930Hu08 | 抗細胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體 | Flow cytometry. |
| MEA930Hu | 細胞外信號調(diào)節(jié)激酶2(ERK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA930Hu | 細胞外信號調(diào)節(jié)激酶2(ERK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA930Hu | 細胞外信號調(diào)節(jié)激酶2(ERK2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Cellular and Molecular Neurobiology | Hypericum Perforatum?Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease [Pubmed:26119304] |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome [pubmed:27538909] |
| International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis [33575317] |
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