無翅型MMTV整合位點家族成員3A(WNT3A)多克隆抗體
Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
- 編號PAP155Bo01
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPP155Bo01-無翅型MMTV整合位點家族成員3A(WNT3A)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Homo sapiens (Human，人)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 832 ¥ 1940 ¥ 2772 ¥ 6930 ¥ 27720
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Skin cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human WNT3A Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: Bovine Small intestine lysate Primary Ab: 5μg/ml Rabbit Anti-Bovine WNT3A Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對WNT3A的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別WNT3A。
用法
Western blotting: 0.01-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPP155Bo01 | 無翅型MMTV整合位點家族成員3A(WNT3A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAP155Bo01 | 無翅型MMTV整合位點家族成員3A(WNT3A)多克隆抗體 | WB; IHC; ICC; IP. |
參考文獻
| 雜志 | 參考文獻 |
| Medical Oncology | Wls promotes the proliferation of breast cancer cells via Wnt signaling [Pubmed:25801232] |
| J Histochem Cytochem | Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs [PubMed: 26209081] |
| PloS one | Gelatin device for the delivery of growth factors involved in endochondral ossification [pubmed:28380024] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618] |
| Oxidative Medicine and Cellular Longevity | Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle [Pubmed:30151071] |
| The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer [Doi: 10.15369/sujms.30.359] |
| Journal of Cancer | Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma [Pubmed: 31737122] |
| CANCER LETTERS | Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection [Pubmed: 32920199] |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties [34823885] |
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